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c20 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc c20 plasmid
    a Balb/c mice were vaccinated with the <t>C20</t> vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).
    C20 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c20 plasmid/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    c20 plasmid - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Neoantigen cancer vaccine augments anti-CTLA-4 efficacy"

    Article Title: Neoantigen cancer vaccine augments anti-CTLA-4 efficacy

    Journal: NPJ Vaccines

    doi: 10.1038/s41541-022-00433-9

    a Balb/c mice were vaccinated with the C20 vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).
    Figure Legend Snippet: a Balb/c mice were vaccinated with the C20 vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).

    Techniques Used: Staining, MANN-WHITNEY

    Immunogenic neoantigens expressed by C20 vaccine.
    Figure Legend Snippet: Immunogenic neoantigens expressed by C20 vaccine.

    Techniques Used: Immunopeptidomics, Enzyme-linked Immunospot

    a Balb/c mice were inoculated s.c. with CT26 cells and treated with C20 and ICI starting from day 2 as depicted in the experimental scheme. Tumor volume and survival curve. b , c Balb/c mice were inoculated s.c. with CT26 cells and treated with ICI and NCV according to the experimental scheme. b Tumor (50–100 mm 3 ) bearing mice were randomized at day 6 and treated with αCTLA-4 and vaccinated with C20 the day after. The treatment was repeated weekly as described in the scheme. b Tumor volume measurements and survival curve. This experiment was conducted only once ( C ) CT26 tumor growth in CD4 or CD8 depleted mice treated as in panel ( b ). This experiment was repeated twice with similar results. Six animals per group were utilized with the error bars representing the s.e.m. Significance was determined using Mann–Whitney and Log-rank (Mantel–Cox) test ** p < 0.01 *** p < 0.001.
    Figure Legend Snippet: a Balb/c mice were inoculated s.c. with CT26 cells and treated with C20 and ICI starting from day 2 as depicted in the experimental scheme. Tumor volume and survival curve. b , c Balb/c mice were inoculated s.c. with CT26 cells and treated with ICI and NCV according to the experimental scheme. b Tumor (50–100 mm 3 ) bearing mice were randomized at day 6 and treated with αCTLA-4 and vaccinated with C20 the day after. The treatment was repeated weekly as described in the scheme. b Tumor volume measurements and survival curve. This experiment was conducted only once ( C ) CT26 tumor growth in CD4 or CD8 depleted mice treated as in panel ( b ). This experiment was repeated twice with similar results. Six animals per group were utilized with the error bars representing the s.e.m. Significance was determined using Mann–Whitney and Log-rank (Mantel–Cox) test ** p < 0.01 *** p < 0.001.

    Techniques Used: MANN-WHITNEY



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    c20 plasmid  (Addgene inc)
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    a Balb/c mice were vaccinated with the <t>C20</t> vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).
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    addgene plasmid  (Addgene inc)
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    a Balb/c mice were vaccinated with the <t>C20</t> vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).
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    Image Search Results


    a Balb/c mice were vaccinated with the C20 vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).

    Journal: NPJ Vaccines

    Article Title: Neoantigen cancer vaccine augments anti-CTLA-4 efficacy

    doi: 10.1038/s41541-022-00433-9

    Figure Lengend Snippet: a Balb/c mice were vaccinated with the C20 vaccine at days 0, 21, and 42 and challenged with CT26 cells on day 62. One week after the last immunization (day 49), mice were bled retro-orbitally to monitor T cell immune response against CT26-neoepitopes by intracellular staining. Panel describes CD8 and CD4 neoantigen-specific effector and central memory T cell responses measured by FC using the gating strategy depicted in Supplementary Fig. . The stimulation pool included the 15 peptides listed in Table . b The panel depicts CT26 tumor growth overtime of one out of two experiments performed. Five animals per group were utilized. Each symbol represents an individual sample with the error bars representing the s.e.m. Significance was determined using Mann–Whitney test (* p < 0,05).

    Article Snippet: M8 and C20 plasmid were deposited in Addgene data base (#80536).

    Techniques: Staining, MANN-WHITNEY

    Immunogenic neoantigens expressed by C20 vaccine.

    Journal: NPJ Vaccines

    Article Title: Neoantigen cancer vaccine augments anti-CTLA-4 efficacy

    doi: 10.1038/s41541-022-00433-9

    Figure Lengend Snippet: Immunogenic neoantigens expressed by C20 vaccine.

    Article Snippet: M8 and C20 plasmid were deposited in Addgene data base (#80536).

    Techniques: Immunopeptidomics, Enzyme-linked Immunospot

    a Balb/c mice were inoculated s.c. with CT26 cells and treated with C20 and ICI starting from day 2 as depicted in the experimental scheme. Tumor volume and survival curve. b , c Balb/c mice were inoculated s.c. with CT26 cells and treated with ICI and NCV according to the experimental scheme. b Tumor (50–100 mm 3 ) bearing mice were randomized at day 6 and treated with αCTLA-4 and vaccinated with C20 the day after. The treatment was repeated weekly as described in the scheme. b Tumor volume measurements and survival curve. This experiment was conducted only once ( C ) CT26 tumor growth in CD4 or CD8 depleted mice treated as in panel ( b ). This experiment was repeated twice with similar results. Six animals per group were utilized with the error bars representing the s.e.m. Significance was determined using Mann–Whitney and Log-rank (Mantel–Cox) test ** p < 0.01 *** p < 0.001.

    Journal: NPJ Vaccines

    Article Title: Neoantigen cancer vaccine augments anti-CTLA-4 efficacy

    doi: 10.1038/s41541-022-00433-9

    Figure Lengend Snippet: a Balb/c mice were inoculated s.c. with CT26 cells and treated with C20 and ICI starting from day 2 as depicted in the experimental scheme. Tumor volume and survival curve. b , c Balb/c mice were inoculated s.c. with CT26 cells and treated with ICI and NCV according to the experimental scheme. b Tumor (50–100 mm 3 ) bearing mice were randomized at day 6 and treated with αCTLA-4 and vaccinated with C20 the day after. The treatment was repeated weekly as described in the scheme. b Tumor volume measurements and survival curve. This experiment was conducted only once ( C ) CT26 tumor growth in CD4 or CD8 depleted mice treated as in panel ( b ). This experiment was repeated twice with similar results. Six animals per group were utilized with the error bars representing the s.e.m. Significance was determined using Mann–Whitney and Log-rank (Mantel–Cox) test ** p < 0.01 *** p < 0.001.

    Article Snippet: M8 and C20 plasmid were deposited in Addgene data base (#80536).

    Techniques: MANN-WHITNEY